Marine bacterial communities in the upper gulf of Thailand assessed by Illumina next-generation sequencing platform

First report of bacterial community from a Bat Guano using Illumina next-generation sequencing

V4 hypervariable region of 16S rDNA was analyzed for identifying the bacterial communities present in Bat Guano from the unexplored cave - Pnahkyndeng, Meghalaya, Northeast India. Metagenome comprised of 585,434 raw Illumina sequences with a 59.59% G+C content. A total of 416,490 preprocessed reads were clustered into 1282 OTUs (operational taxonomical units) comprising of 18 bacterial phyla. T...

An Introduction to Illumina Next-Generation Sequencing Technology for Microbiologists

An Introduction to Illumina Next-Generation Sequencing Technology for Microbiologists

Deciphering DNA sequences is essential for virtually all branches of biological research. Capillary electrophoresis (CE)-based sequencing has enabled scientists to elucidate genetic information from almost any organism or biological system. Although this technology has become widely adopted, inherent limitations in throughput, scalability, cost, speed, and resolution can hinder scientists from ...

Strategies and Clinical Applications of Next Generation Sequencing

Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput se­quencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...

Next Generation Sequencing and its Application in the Study of Microbiome in Plant Diseases Suppressive Soils

Progress in next-generation sequencing has played a significant role in ecological studies of microbial populations. These advances have led to a rapid evaluation in metagenomics studies (analysis of DNA of microbial communities without the need to culture). Many statistical and computational tools and metagenomics databases have led to the discovery of huge amounts of data. In this research, i...